dc- sign mouse antibody  (Santa Cruz Biotechnology)

Journal: iScience

Article Title: Macrophages treated with interferons induce different responses in lymphocytes via extracellular vesicles

doi: 10.1016/j.isci.2024.109960

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Article Snippet: Mouse monoclonal anti CD209/DC-SIGN (clone REA617) Conjugated APC , Miltenyi , Cat#130-124-257.

Techniques: Recombinant, Protein Extraction, Protease Inhibitor, Pore Size, Electrophoresis, Staining, Software, Cytometry

Journal: International journal of molecular sciences

Article Title: Analysis of CD1a-Positive Monocyte-Derived Cells in the Regional Lymph Nodes of Patients with Gallbladder Cancer.

doi: 10.3390/ijms252312763

Figure Lengend Snippet: Figure 2. Representative images of immunohistochemistry of CD209 (DC-SIGN) ((a) original mag- nification ×100, (b) original magnification ×200). (a) Many CD209-positive cells are found at the sinuses of LNs. (b) In metastatic LNs, CD209-positive cells are found at stroma surrounding cancer cells, some of them having dendritic shapes. (c) Image of immunohistochemistry of CD1a, almost the same area of (b). Dendritic-shaped CD1a-positive cells are found at stroma surrounding cancer cells (original magnification ×200).

Article Snippet: The following primary antibodies were used: the mouse monoclonal anti-CD1a antibody (clone 010; IS06930–2; prediluted; Dako, Glostrup, Denmark); the rabbit polyclonal antiS100 antibody (GA50461–2 J; prediluted; Dako); and the mouse monoclonal anti-CD209 antibody (clone 120507; R&D systems, Minneapolis, MN, USA).

Techniques: Immunohistochemistry

Journal: iScience

Article Title: Muscle glycome in idiopathic inflammatory myopathies: Impact in IL-6 production and disease prognosis

doi: 10.1016/j.isci.2023.107172

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Article Snippet: Subsequently, non-specific binding was prevented by blocking with 2% mouse serum in FACS for 10 min and the biotinylated lectin was incubated with streptavidin-PE-Cy7 (eBioscience) for 30 min. For surface marker staining, cells were stained for 30 min on ice and protected from light with the following antibodies: BV421 anti-human CD14 (clone 63D3), PE -Cy7 anti-human CD206 (clone 15-2) from Biolegend; eFluorTM 450 anti-human CD4 (clone RPA-T4), APC anti-human CD11c (clone BU15), from eBioscience; PE anti-human TCRα/β (clone BW242/412) from Miltenyi; BV510 anti-human CD3 (clone OKT3) from BD Biosciences; for DC-SIGN staining, cells were incubated with polyclonal DC-SIGN rabbit IgG (Biorad), followed by incubation with polyclonal porcine anti-rabbit IgG (FITC; Dako) for 30 min on ice.

Techniques: Plasmid Preparation, Recombinant, Reverse Transcription, Random Hexamer, Protease Inhibitor, Staining, Isolation, Enzyme-linked Immunosorbent Assay, Software